...
_____________________________________________________________________
_____________________________________________________________________
Sampling time after treatment
_____________________________________________________________________
...
Sample Cells/tissue description (gender, type of cells, genetic manipulation, FAX, treatment..)
Species ___________________________________________________________________
Gender____________________________________________________________________
___________________________________________________________________________
Tissue description____________________________________________________________
_____________________________________________________________________________
_____________________________________________________________________________
Cell types____________________________________________________________________
_____________________________________________________________________________
Genetic Manipulation____________________________________________________________
Estimating replicates needed
...
_____________________________________________________________________________
_____________________________________________________________________________
Lab manipulation (FAX, nuclei)_____________________________________________________
_____________________________________________________________________________
...
Other information_______________________________________________________________
Estimating replicates needed
- Sample Purity (% of cells which are of interest, reproducibility of this percentage)
_________________________________________________________________________
- Genetic purity cell line (at least 3 replicates)
whole organism inbreed (at least 3 replicates) other (even 10 replicates)
_________________________________________________________________________
- Pooling – avoid if possible. At least 5 samples. Need to have 3 replicates.
_________________________________________________________________________
- If there is small variance between treatments more replicates needed yes no
...
For single cell analysis estimation of abundancy of cell population of interest (in percent)
______________________________________________________________________________
______________________________________________________________________________
Summary:
Platform name: ILLUMINA PACBIO NANOPORE MS
Number of groups: __________ Number of replicates: __________
Total number of samples: __________
Type of Illumina design: PE SR
Length of the read _________
Number of reads_________________________________________________________________
Protocol type _______________________________
Summary:
Platform name: ILLUMINA PACBIO NANOPORE MS
Number of groups: ________________ Number of replicates: __________Total number of samples: __________
Type of Illumina design: PE SR
Length of the read
Duration for first analysis:
Evaluation of project size for bioinformatics analysis: Low/Medium/Large
Duration for initial analysis :_________________________________ Number of reads _____Protocol type _______
Duration for first analysis:
Evaluation of project size for bioinformatics analysis: Low/Medium/Large
Duration (Relative to data received date):
Expected date when the sequences will be ready :______________________________________