The "Demultiplexing from RUNID" pipeline divides the reads among the samples, and prepares FASTQ files for each sample.
Pipeline website: http://utap.wexac.weizmann.ac.il
Before you start:
This pipeline runs on the Wexac cluster.
Please prepare the following in advance:
Setting up a new analysis
Note: This pipeline is only for users whose sequencing was done at the WIS Sandbox unit and hence have bcl files on the Stefan server. Need to check - Jordana/Michal
The pipeline copies the bcl files from the Stefan server into your Collaboration folder on Wexac, converts them to fastq files, and demultiplexes the fastq files according to the selected MARS-Seq or TruSeq (default) protocol.
You need to supply only the run id of your sequencing run (e.g.: 170802_NB501465_0140_AH3W3KBGX3). You can find this id in the email that you receive upon completion of the sequencing run.
Click on Demultiplexing_from_RUNID in the Choose pipeline box:
At the end of the run, a folder containing your samples' fastq files will be created in the Output folder that you specify.
/** What about dual barcode (index)? */
Transcriptome pipeline for Weizmann Institute users: http://utap.wexac.weizmann.ac.il
Kohen et al. BMC Bioinformatics (2019) 20:154 https://doi.org/10.1186/s12859-019-2728-2 (PMID: 30909881)